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10mm dntp mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 10mm dntp mix
    10mm Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10mm+dntp+mix/fetal+bovine+serum/pm39216481-342-24-26
    Average 90 stars, based on 1 article reviews
    10mm dntp mix - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Purification:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Lysis:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    cDNA Synthesis:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Amplification:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Sequencing:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Isolation:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Reverse Transcription:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Concentration Assay:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Spectrophotometry:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Recombinant:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    SYBR Green Assay:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Staining:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.

    Control:


    Article Title: Cartography of the developability landscapes of native and human-engineered antibodies
    Article Snippet: RNA quality and concentration were measured using a Nanodrop spectrophotometer (Thermo Fisher Scientific). (2) cDNA synthesis 200 ng of RNA was used for cDNA synthesis with 1 μl of 100 μM isotype-specific reverse primers, 1 μl of 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water up to 14.5 μl.

    Article Title: Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos.
    Article Snippet: Article Maternal KLF17 controls zygotic genome activation by acting as a messenger for RNA Pol II recruitment in mouse embryos

    Article Title: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
    Article Snippet: From isolated RNA, 200 ng was used to synthesize cDNA using 1 μl 100 μM isotype-specific reverse transcription primers , 1 μl 10mM dNTP Mix (Thermo Fisher Scientific) and nuclease-free water to a final volume of 14.5 μl.

    Article Title: Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance.
    Article Snippet: A cc ep te d A rt ic le It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood.. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection.. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned.

    Article Title: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
    Article Snippet: Cleaved DNA ends were repaired by adding 1 μL T4 DNA polymerase (Thermo Fisher Scientific), 1 μL 10mM dNTP mix (Thermo Fisher Scientific) and incubating at 11 °C for 20min.


    Article Title: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.
    Article Snippet: DNase-treated total RNAwas thenmixedwith 0.1μg of random hexamers, 0.6μg of oligo-dT (20), and 2μL 10mM dNTP mix (Life Technologies) in total volume of 10μL, incubated for 10minutes at 70 °C and then chilled on ice for 5minutes.



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